CNR4All Misura simultanea di marcatori della sepsi in pazienti in terapia intensiva
A fluorescence-based optical platform for the simultaneous measurement up to four different biomarkers in serum or plasma samples has been developed; an optical chip with thirteen different channels is the heart of the instrument. Fluorescence detection is performed by means of the implementation of a sandwich assay with the secondary antibody properly labelled with a fluorophore. Attention was focused on procalcitonin (PCT), C-reactive protein (CRP), interleukin-6 and soluble urokinase plasminogen activator receptor (suPAR), with suPAR being considered in the last years as an important prognostic biomarker for sepsis. Two measurement campaigns have been carried out with the developed device at the Attikon University Hospital (Athens, Greece) and at Center for Sepsis Control and Care of the Universitätsklinikum Jena (Jena, Germany).
Sepsis is the highest cause of mortality in intensive care units and is surely one of the pathologies for which the concept of Point of Care Testing - the capability of performing the measurements of the analytes of clinical interest close to the patient bed and not in central laboratories - is better appropriate. As a matter of fact, it has been demonstrated that delays of also a few minutes until administration of an effective antibiotic treatment in septic shock, can lead to a significant increase of mortality if the origin of the inflammatory process is bacterial. The fast discrimination between viral and bacterial sepsis and, in case, the immediate identification of the correct antibiotic therapy is fundamental for the patient survival. In the complex clinical picture of septic patients, biomarkers can provide supportive and supplemental data to clinical assessment.
Francesco Baldini responsible of the Chemical & Biochemical Optical Sensor Group; Romeo Bernini (IREA)
Francesco Baldini, graduated in physics from the University of Florence magna cum laude in 1986, joined the Optical Fiber Group at IROE-CNR (now IFAC-CNR) in Florence in 1986. In the first years of activity, his research was devoted to the development of optical fibre sensors and to the application of optical methods to the restoration of paintings and frescoes. Since the beginning of the Nineties, he is active in the field of optical sensors and devices for chemical and biochemical parameters, mainly for biomedical application. He is author of more than 200 publications on the subject in International Journals, in scientific books and in International Conference Proceedings and delivered more than 30 invited presentation at International and National conferences. In 2009 he was nominated fellow of SPIE for his achievements in biological and chemical sensing in biomedicine. He has been President of the Italian Society of Optics and Photonics (SIOF) for the biennium 2015-2016. He is/was coordinator and/or responsible of many international and national projects in the field of optical chemical and biochemical sensors. He was guest editor of a special issue of Sensors & Actuators on the Europt(r)ode II Conference held in Florence in 1994 and of several issues in Analytical and Bioanalytical Chemistry. He is associated editor of Journal of Sensors and he was member of the International Advisory Board of the Journal Analytical & Bioanalytical Chemistry from 2005 to 2013. He is Chairman of the ASCOS (Advanced Study Course on Optical Chemical Sensors) Series (www.ascos.org) since 2010 and he was the chairperson of many international Conferences. He is a member of the Steering Committee of the Europt(r)ode Conference on Optical Chemical Sensors and Biosensors and is/has been member of the Scientific Committee of many International and National Conferences.
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Consiglio Nazionale delle Ricerche CNR4All#